Journal: Cell reports
Article Title: Catecholaminergic dysfunction drives postural and locomotor deficits in a mouse model of spinal muscular atrophy
doi: 10.1016/j.celrep.2024.115147
Figure Lengend Snippet: (A) Schematic illustration showing the EMG electrodes in the tibialis anterior (TA) muscle bilaterally during in vivo voluntary locomotion. (B–G) Bilateral TA EMG activity in a WT (B), SMA (C), SMA::TH Cre (D), SMA::Pet1 Cre (E), SMA + ( l -dopa + B) (F), and SMA::(TH + Pet1) Cre (G) mice during freely moving locomotion in vivo at P11. Bottom traces show the raw recordings, and upper traces display the integrated (rectified and filtered) signals. The dotted vertical red lines show the beginning of each burst of TA activity in both legs determined by the integrated signal. Each cycle is defined by the time between two consecutive vertical red lines. In addition, the corresponding polar plots indicate the right and left alternation. Each dot corresponds to the phase shift of each cycle compared to the cycle in the opposite leg for a representative mouse in each group. The number of cycles shown in (B)–(G) plots analyzed are WT, n = 85 cycles; SMA, n = 104; SMA::TH Cre , n = 103; SMA::Pet1 Cre , n = 108; l -dopa and benserazide, n = 78; SMA::(TH + Pet1) Cre , n = 99. The bar corresponds to the mean vector r. The vector length represents the left-right alternation strength, and the vector angle indicates the average alternation phase shift between the left and right TA EMG. **** p < 0.0001; Rayleigh test. (H) The angular dispersion represents the variability of the left-right alternation between the two hindlimbs from WT (black, N = 6 mice), SMA (red, N =6 mice), SMA::TH Cre (blue, N = 6 mice), SMA::Pet1 Cre (orange, N = 5 mice), SMA + ( l -dopa + B) (purple, N = 6 mice), and SMA::(TH + Pet1) Cre (green, N =6 mice). ** p < 0.01; *** p < 0.001; one-way ANOVA, Tukey’s post hoc analysis. (I) Average cycle period during locomotion in each mouse for the same groups and number of mice shown in (H). * p < 0.05; one-way ANOVA, Tukey’s post hoc analysis. (J) Average burst duration for the same groups and numbers of mice shown in (H).
Article Snippet: A pair of thin (0.003′′ bare; 0.0055′′ coated; A-M Systems) Teflon-coated silver wire electrodes were inserted 0.5 mm deep and sutured in the belly of each tibialis anterior muscle (2–3 mm between each electrode).
Techniques: In Vivo, Activity Assay, Plasmid Preparation, Dispersion
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